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For in-use pens that have already been stored at room temperature as per guidance, an additional overnight exposure is less concerning, provided the cumulative room-temperature storage does not exceed 6 weeks

All experiments involving the collection and evaluation of COCs, in vitro maturation and fertilization of oocytes, and the assessment of fertilized oocytes, embryos and cumulus cells were conducted following the procedures previously described by our working group 22,23,24

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