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Male C57BL/6 mice (10 weeks old) were anesthetized with isoflurane, and mesenteric arteries were then isolated and placed in ice-cold KrebsHenseleit solution, containing 112 mM NaCl, 5 mM KCl, 25 mM NaHCO 3 , 1 mM NaH 2 PO 4 , 0.5 mM MgCl 2 , 2.5 mM CaCl 2 , and 11.5 mM glucose

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Conversely, in a recent randomized, double-blind, placebo-controlled study involving twenty healthy volunteers, a once-weekly injection of a GLP-1R agonist, dulaglutide, for three weeks did not influence HPA axis activity, showing no significant effects on circadian rhythms of blood or salivary cortisol secretion, and the level of this hormone also was not changed after the dexamethasone suppression test in comparison with the placebo-receiving group [78]