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2014;3:e03421

A single Q227E mutation introduces ADH activity in an archaeon TyrA p To test if the non-acidic residue of MhTyrA p at the corresponding 222 position (Gln227) is involved in prephenate substrate specificity, site-directed mutagenesis was performed on MhTyrA p to replace Gln227 with acidic Glu and generate the MhTyrA p Q227E mutant

Antipsychotic drug and body weight set-point

iGL cells, used for the evaluation of glucose-stimulated insulin secretion (GSIS), were purchased from CosmoBio and cultured in RPMI-1640 (Wako) containing 10% FBS

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