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Since glycolysis is the only way to produce adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide hydrogen (NADH) energy in the RBCs, lactic acid produced in glycolysis leads to a decrease in pH during six weeks of storage

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For this purpose, we utilized 4-hydroxy-2-nonenal (4-HNE), which is one of the major products of lipid peroxidation and can be used as a marker for oxidative stress 11,12,13

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