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The membranes were blocked and then incubated with anti-ELANE (ab131260, Abcam), anti-CD14 (DF13278, Abcam), anti-S100A11(ab169530, Abcam), anti-PGAM1(DF12693, affinity), anti-SERPINB10(DF9894, affinity), anti-BST2 (DF3846, affinity), and anti--actin (AF7018, affinity) overnight at 4C

Figure 4 Restriction digestion analysis of pET24-His6-AV-Ubiquitin-Semapeptide Gene (clones)

Abstract Background and Objective Tirzepatide, a novel, once-weekly, dual glucose-dependent insulinotropic polypeptide and glucagon-like peptide-1 receptor agonist, is approved in the US as a treatment for type 2 diabetes and is under development for long-term weight management, heart failure with preserved ejection fraction, and nonalcoholic steatohepatitis